Genetic characteristics of VP1 of coxsackievirus A24 associated with hand foot and mouth disease in Northern provinces, Vietnam, 2013 - 2014

Abstract

Coxsackievirus A24 (CV-A24) is a major causative agent involved in acute hemorrhagic conjunctivitis (AHC) but it was recently reported to be detected from patients with Hand foot and mouth disease (HFMD) in China between 2013 and 2014. While conducting HFMD surveillance in Northern Vietnam, CVA24 was also detected in 2.4% (13/545) during
2013 and 2014. This study aims to characterize
VP1 gene of coxsackievirus A24 associated with hand foot and mouth disease in Northern provinces, Vietnam, 2013 - 2014. Phylogenetic analysis revealed that all Vietnamese CVA24 belonged to subgenogroup GIV-5, the largest subgenogroup comprising CV-A24v isolated from patients with AHC worldwide. The VP1 nucleotide sequence identities among Vietnamese CV-A24 ranged from 97.4% to
100%; those sequences were mostly closed with Thailand CV-A24 isolates causing AHC in 2014
with the nucleotide identities of 96.7% - 99.3%.
There was no informative amino acid mutation on the VP1 of CV-A24v isolates among the AHC and HFMD groups or among the HFMD severe (2b) and moderate (1-2a) groups. This is the first study on genetic characteritis of CVA24 associated with HFMD, providing further evidence in the change of clinical manifestation to be etiological agent of HFMD as reported in previous studies of CV-A6, CV-A2, and
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