Identification of some species of house dust mites isolated in Hanoi, Vietnam using molecular biology

Abstract

This study aimed to evaluate the feasibility of identifying three house dust mite (HDM) species, Blomia tropicalis, Dermatophagoides pteronyssinus, and Dermatophagoides farinae, isolated in Vietnam using various molecular biology methods. The research was conducted on samples of these three HDM species collected from 970 house dust samples around Hanoi. The results indicated that molecular biology methods produced similar outcomes to morphological identification. The phylogenetic tree construction revealed that the ITS2 sequences of B. tropicalis, D. pteronyssinus, and D. farinae were closely related to the ITS2 sequences of the respective species previously published. Identification PCR reactions demonstrated that HDM species can be easily identified and differentiated based on PCR product size, with no cross-products generated. Specifically, PCR products were 250 bp for B. tropicalis, 650 bp for D. pteronyssinus, and 750 bp for D. farinae. These findings confirm that B. tropicalis, D. pteronyssinus, and D. farinae isolated in Vietnam can be accurately identified using ITS2 sequencing and speciesspecific PCR methods.