Improvement of production proceduces of rubella bulk in Vietnam

Abstract

Center for Research and Production of Vaccines and Biologicals (POLYVAC) has studied aims to optimize the rubella bulk
production procedures based on technical transfered procedures from Japan. The results showed that: Suitable concentration of cells is 13 × 104 (cells/ml), at this cell concentration, vaccine potency is stable, reduce production cost, suitable time in production. Harvest of vaccine at 14th day from cell culture time or at 4th day from time of washing cell bottles and
changing medium. For vaccine thawing and distribution procedures, we already improved from harvest – pooling – adding stabilizers - clarifying – distributing to 10L stainless steel tanks → quickly freezing → stored at ≤ -650 C to new procedures: Harvest – pooling – adding stabilizers - distributing to 10L stainless steel tanks – quickly freezing – stored at ≤ -650 C → thawing → pooling – clarifying – distributing to 10L stainless steel tanks → quickly freezing → stored at ≤ -650 C.