Characteristics of antibiotic resistance of mcr-1 carrying E.coli strains isolated from humans, animals, foods and water in Thanh Ha commune, Thanh Liem district, Ha Nam province in 2015

Abstract

Facing the current serious situation of antibiotic resistance, colistin is an important priority antibiotic used for carbapenem - resistant gram - negative intestinal infections. The study aimed to describe characteristics of antibiotic resistance of mcr-1-carrying E.coli strains isolated from humans, animals, foods and water in Thanh Ha commune, Thanh Liem
district, Ha Nam province in 2015. Research results showed that the rate of E.coli strains carrying mcr-1 gene in healthy people was 36.6%; In domestic animals it was 34.4%; in water it was 8.4% and in food it was 3.8%. Among 398 E.coli strains carrying the mcr-1 gene, 160 strains had MIC values from 4 - > 16µg/ml, 80% of these strains had MIC = 4µg/ ml. E.coli strains carrying the mcr-1 gene had high rates of resistance to commonly used antibiotics such as β-lactams, aminoglycosides, quinolones, and SXT including a number of multi - resistant strains, resistance to ampicillin (lowest 97.6% - highest 100%), gentamicin (lowest 29.2% - highest 49.4%), ciprofloxacin (lowest 31.3%-highest 81.2%) and SXT
(lowest 71.7% and highest 96.9%) ). Among them, there are 115 (41.3%) multi-resistant strains, resistant to 2-3 antibiotics or more, 07 strains resistant to the entire β-lactam group. 46 types of AMR genes have been found in which many genes are located on plasmids resistant to important antibiotic groups.