Detection of virulence genes of Escherichia coli strains isolated from healthy people in Nguyen Xa commune, Vu Thu district, Thai Binh province by Multiplex PCR

Abstract

Escherichia coli (E. coli) is a bacteria that lives symbiotically in the human gastrointestinal tract but is also one of the main causes of diarrhea. Across - sectional descriptive study was conducted to detect the virulence genes of E. coli strains isolated from healthy people in Nguyen Xa commune, Vu Thu district, Thai Binh province, by multiplex PCR. The results showed that the rate of E. coli strains carrying virulence genes was 41.4%. Analyzing 169 E. coli strains, the study detected 8 different virulence genes, the most common of which was the astA gene (29.6%). Each E. coli strain could carry 1 to 3 virulence genes. Among E. coli strains carrying virulence genes, the EAEC group is the most common (20.7%), followed by the EPEC group (13.0%), while the ETEC, EAEC/DAEC and EAEC/DAEC groups account for very low rates.