Preparation of His-Tagged Armored RNA particles as positive controls for Real-Time RT-PCR Detection of Zika virus

Các tác giả

  • Do Thi Quynh Nga National Institute of Hygiene and Epidemiology, Hanoi
  • Ha Thi Phuong Mai National Institute of Hygiene and Epidemiology, Hanoi
  • Tran Thi Hai Au National Institute of Hygiene and Epidemiology, Hanoi
  • Vu Thi Kim Lien National Institute of Hygiene and Epidemiology, Hanoi
  • Vu Thi Bich Hau National Institute of Hygiene and Epidemiology, Hanoi
  • Nguyen Thanh Thuy National Institute of Hygiene and Epidemiology, Hanoi
  • Pham Van Chung National Institute of Hygiene and Epidemiology, Hanoi
  • Tran Le Tien Ha Dong Hospital, Hanoi
  • Nong Thi Thanh Van Center for reseach and production of vaccines and biologicals, Hanoi
  • Nguyen Thi Thu Thuy National Institute of Hygiene and Epidemiology, Hanoi

DOI:

https://doi.org/10.51403/0868-2836/2021/332

Từ khóa:

Armored RNA technology, positive control, MS2 bacteriophage, zikavirus

Tóm tắt

Armored RNA (AR) is a good candidate for creating nuclease-resistant RNA positive controls in the nucleic acid - based assay for RNA viruses. To simplify the production and purifcation of armored RNA, a single plasmid double – expressing His6-tag system was designed. His-tag armored RNA particles were purifed using his-tag affnity. A genomic fragment of the zika virus consisting of the encoding sequences of flavi-M, flavi-E-C protein targeted for zika virus was selected to prepare a positive control. In this study, we have successfully produced His-taged MS2- phage like particles carrying specifc genomic regions (M and E genes) to monitor the procedures of real-time Reverse transcription-PCR for Zika virus detection in one plasmid double expression. AR-ZIKA is completely resistant to DNase and RNase, stable in normal human EDTA plasma at room temperature for at least 60 and 15 days at 40C and room temperature respectively.

Tải xuống

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Đã Xuất bản

18-06-2021

Cách trích dẫn

Nga, D. T. Q. ., Mai, H. T. P. ., Au, T. T. H. ., Lien, V. T. K. ., Hau, V. T. B. ., Thuy, N. T. ., Chung, P. V. ., Tien, T. L. ., Van, N. T. T. ., & Thuy, N. T. T. . (2021). Preparation of His-Tagged Armored RNA particles as positive controls for Real-Time RT-PCR Detection of Zika virus. Tạp Chí Y học Dự phòng, 31(4), 18–27. https://doi.org/10.51403/0868-2836/2021/332

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