Detecting SARS-CoV-2 by Realtime RT-PCR in clinical samples collected in the North of Vietnam

Các tác giả

  • Hoang Vu Mai Phuong National Institute of Hygiene and Epidemiology, Hanoi
  • Ung Thi Hong Trang National Institute of Hygiene and Epidemiology, Hanoi
  • Nguyen Vu Son National Institute of Hygiene and Epidemiology, Hanoi
  • Le Thi Thanh National Institute of Hygiene and Epidemiology, Hanoi
  • Nguyen Le Khanh Hang National Institute of Hygiene and Epidemiology, Hanoi
  • Vuong Duc Cuong National Institute of Hygiene and Epidemiology, Hanoi
  • Tran Thi Thu Huong National Institute of Hygiene and Epidemiology, Hanoi
  • Pham Thi Hien National Institute of Hygiene and Epidemiology, Hanoi
  • Nguyen Phuong Anh National Institute of Hygiene and Epidemiology, Hanoi
  • Nguyen Co Thach National Institute of Hygiene and Epidemiology, Hanoi
  • Le Thi Quynh Mai National Institute of Hygiene and Epidemiology, Hanoi

DOI:

https://doi.org/10.51403/0868-2836/2020/114

Từ khóa:

SARS-CoV-2, real-time RT-PCR, North of Viet Nam

Tóm tắt

From January to August 2020, Northern Viet Nam faced a COVID-19 outbreak, up to September 2020, there were 1122 confrmed cases of SARS-CoV-2, of which 465 cases were imported from Europe, America and Asia, 657 cases were identifed domestically. A total of 30,686 samples were collected during the SARS-CoV-2 outbreak in Northern Viet Nam and examined by Real-time RT-PCR using primers and probe from Charite - Berlin protocol. This study showed the initial results of SARS-CoV-2 detection and RNA quantitative in positive samples. The positive rate was 0.8%, ranging from 0.4 to 3.5% according to collection sites. Out of 251 positive samples, the mean Ct value was 28 (IQR: 22.3-32; range 14 - 38). The positive samples had a Ct value below 30 was 68.5%, there was no signifcant difference between the Ct value of the group ≤ 30 and > 30. The mean of the RNA copies/µl was 8.4.107, (IQR: 2.29.106 - 1.83.109 RNA copies/µl, range: 1.95.103 – 4.95.1011). In the group of imported COVID-19 cases, the rate of virus at low level was 29%, an average was 56% and at high level was 15%. In the community groups, the viral load data showed that the average rate at low, intermediate and high level were 20%, 63% and 17% respectively. The proportion of high-level viral load may raise an alert to start the quarantine process to reduce the transmission of SARS-CoV-2

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Đã Xuất bản

25-04-2021

Cách trích dẫn

Phuong, H. V. M. ., Trang, U. T. H. ., Son, N. V. ., Thanh, L. T. ., Hang, N. L. K. ., Cuong, V. D. ., Huong, T. T. T. ., Hien, P. T. ., Anh, N. P. ., Thach, N. C. ., & Mai, L. T. Q. . (2021). Detecting SARS-CoV-2 by Realtime RT-PCR in clinical samples collected in the North of Vietnam. Tạp Chí Y học Dự phòng, 30(9), 11–17. https://doi.org/10.51403/0868-2836/2020/114

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