Determination of related genetic variations resistance to Moxifloxacin from Mycobacterium tuberculosis strain in Hanoi from 2020 - 2022

Abstract

The risk of resistance to tuberculosis drugs is a global problem that affects health safety and the goal of ending tuberculosis by 2035. Drug resistance of tuberculosis bacteria involves the emergence of unique mutations. randomly in genes located in the nucleus of bacterial cells. This study applies the MTB whole genome sequencing (WGS) method to analyze mutations related to TB drug resistance to Moxifloxacin (MFX). The results of the study were to isolate 82 strains of M. tuberculosis with complete phenotypic antibiogram results and whole genome sequencing, of which 16 strains were resistant to MFX. The gyrA gene sequencing results showed that 37.5% (6/16) of MFX-resistant strains carried at least 1 MFX resistance mutation and only 1.5% (1/66) of MFX-sensitive strains carried a resistance mutation. The most common mutations detected was in the gyrA_p.Asp94Gly gene (67%), the mutation only occurred in lineage 2 - Beijing related to drug resistance. Molecular diagnosis based on gyrA gene mutations has a sensitivity of 37.5% and a specificity of 98.5%. This is an important basis for considering the application of gene sequencing to quickly detect drug-resistant M. tuberculosis, moving towards individualizing tuberculosis treatment nationwide.