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Page: 63
Volume XXVI, Issue 12(185) 2016
Direct PCR for detection of Helicobacter pylori from clinical samples in Vietnamese pediatric patients
Author: Nguyen Thi Thanh Ngan, Nguyen Thi Ut, Nguyen Thuy Tram, Pham Thanh Hai, Luong Minh Hoa and Hoang Thi Thu Ha
Summary:
This study was to diagnose and genotype Helicobacter pylori (H. pylori) infection in Vietnamese pediatric patients by using direct PCR. Clinical samples were studied from 120 children with gastrointestinal complaints by using culture and antigen-in-stool (HpSA) methods. Direct uni-and multiplex PCRs were performed to target the 16S rDNA, cagA gene and vacA genotypes. Among 120 biopsy samples, culture could detect 79.2% (95/120) as H. pylori. By a direct biopsy-PCR, the 16S rDNA, cagA, and VacA were identified as the most common genes in 98.9%, 97.9% and 100% patients, respectively while cagA gene was detected in 48.4% patients. In parallel, a direct stool-PCR showed 90.5% patients carried 16S rDNA gene, 87.3% vacA and 49.9% cagA gene. The prevalence of vacA s1 and vacA m1 were high in all patients. Multiplex PCR detected the s1m1 genotype as commonly isolated variant, representing 69.4 % of sample positives, among which 34.7% patients were carrying cagA gene. The s1m2 genotype was also ascertained to be frequent (29.8 %). In the post-treatment phase, stool-PCR detected 30 positive among 32 HpSA positive patients. Two (6.7%) patients presented cagA gene in combination with vacA s1/m1 or s1/m2. Direct biopsy-PCR found VacA as the most common gene and vacA s1 emerged as the dominant factor in gastritis, whereas vacA s1 combined with cagA was present in 100% ulcer Vietnamese pediatric patients. Stool-PCR can be a valuable tool for detection of H. pylori infection in Vietnamese pediatric patients – a high risk population.
Keywords:
Direct PCR, genotyping H. pylori, pediatric patients, Vietnam
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Happy New Year 2019! Year of the Pig
The Vietnam Journal of Preventive Medicine wish all readers with healthy, happiness and prosperity in the year of the Pig. Happy New Year 2019
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